Journal article
Mammary cell-activating factor regulates the hormone-independent transcription of the early lactation protein (ELP) gene in a marsupial
EA Pharo, MB Renfree, KN Cane
Molecular and Cellular Endocrinology | ELSEVIER IRELAND LTD | Published : 2016
Abstract
The regulation of the tammar wallaby (Macropus eugenii) early lactation protein (ELP) gene is complex. ELP is responsive to the lactogenic hormones; insulin (I), hydrocortisone (HC) and prolactin (PRL) in mammary gland explants but could not be induced with lactogenic hormones in tammar primary mammary gland cells, nor in KIM-2 conditionally immortalised murine mammary epithelial cells. Similarly, ELP promoter constructs transiently-transfected into human embryonic kidney (HEK293T) cells constitutively expressing the prolactin receptor (PRLR) and Signal Transducer and Activator of Transcription (STAT)5A were unresponsive to prolactin, unlike the rat and mouse β-casein (CSN2) promoter constru..
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Funding Acknowledgements
This research was funded by the Cooperative Research Centre for Innovative Dairy Products (CRC-IDP) and the Department of Zoology, The University of Melbourne, Australia. EAP was the recipient of an Australian Postgraduate Award (APA) and KNC, a Dairy Australia Postgraduate Student Award and both EAP and KNC received a scholarship top-up from the CRC-IDP. We thank Professor Kevin Nicholas for his helpful advice, Dr. Hong-Jian Zhu for the use of his GloMax-96 Microplate Luminometer, Josie laria for her assistance with dual luciferase assays and Sonia Mailer for assistance with the preparation of tammar PMGCs.